PGD/PGS
We are happy to announce the availability of the PGD/PGD mentioned tests at Lush Fertility.
We discuss all considerations on an individual basis with the patient giving utmost attention to all factors involved in any fertility issue.
Before making any decision about genetic testing, we review each couples history and offer the most viable option to them.
Preimplantation genetic diagnosis (PGD) and Pre-Implantation genetic screening (PGS) are tests done on embryos for a particular genetic disease that it may be at risk of inheriting from the parents.
Biopsy is carried out for PGD/PGS.
Embryo Biopsy
Preimplantation Genetic Diagnosis screens an embryo’s quality at the chromosomal and genetic level. It can be performed for a patient at high risk of carrying an inheritable genetic disease. Using this technique, embryos that do not carry the disease can be selected for transfer.
A single cell from an 8-cell stage embryo is removed and sent off for testing. DNA analysis identifies embryos that are free of the disease, making them good candidates for transfer.
Why is Embryonic selection beneficial?
For patients with genetic diseases, selection of embryos most likely to implant and develop into a healthy child becomes extremely important. Historically, the microscopic appearance of the embryo was the only criteria available to the physician and patient for selection of “healthy” embryos for transfer. Unfortunately, there is no correlation between embryonic appearance and genetic health. Perfect-appearing embryos can carry chromosomal abnormalities and “unattractive” embryos can be genetically normal. The advent of PGD gave physicians and their patients with genetic disease an effective
Clinical indications for which PGD can be used include, but are not limited to:
Aneuploidy testing for advanced maternal age and recurrent pregnancy losses(duplicated or missing chromosomes
Embryo Biopsy for PGD and PGS The process of IVF PGD as well as IVF PGS can be performed on the embryos that are in different developmental stages, therefore the biopsy procedure varies accordingly. In clinical practice, only two types of biopsy are used:
- Blastomere biopsy– (on day three at cleavage stage embryos) – 1 or 2 cells are removed from 8-cell embryo for genetic analysis
- Trophoectoderm biopsy– (on day five at blastocyst stage embryos) – 4 or 5 cells are taken from the outer trophectoderm layer without affecting the inner cell mass from which the fetus later develops.
The Biopsy procedure always involves two steps. The first is the opening of the zona pellucida and the second being removal of the cell(s). Both the steps have different approaches, including mechanical as well as chemical procedures, but most advanced laser technology is used for breaching the zona pellucida
Using the advanced technology, the zona thinning is possible to create a point of herniation already at the time of ICSI procedure, that is less disturbing for embryo than traditional zona-opening. During trophoectoderm (TE) biopsy we operate with accurately controlled laser beam to release the outer cells of the blastocyst.
The retrieved embryonic cells are then sent for genetic analysis by the use of microchip method. In this a larger number of cells allows a more accurate genetic assessment of the embryo without affecting its further development. Once the cells have been extracted from a blastocyst, the embryos are then frozen and stored for a few days until the genetic results are available. The main limitation of the process of blastocyst biopsy is that usually only a limited number of embryos will reach the blastocyst stage. Therefore, the final decision on biopsy approach should be left to our experienced specialists.
The benifits of PGD are as follows:
PGD canb test for more than 100 different genetic conditions
PGD is performed before implantation thereby offering the couple the option to decide whether or not they choose to go ahead with the pregnancy
PGD greatly reduces the risk of passing on specific genetic diseases to their biological offspring
Other Treatments
IVF/ICSI With Donor Sperm
ICSI with donor sperm involves the fertilization of the extracted eggs (from the Patient )...
Read MoreFertility Preservation
What is Fertility preservation ? Fertility preservation is the technique of conserving or storing one’s...
Read MoreTesticular Biopsy
A testicular biopsy is a surgical procedure where a tissue sample from your testicle is...
Read MoreHydrocele Treatment
A hydrocele Treatment is a collection of serous fluid between the two layers of the...
Read MoreVaricocelectomy
Diagnosis Your doctor will conduct a physical exam, which might reveal a nontender mass above...
Read MoreTESA/TESE/PESA/MESA
TESA /TESE ( Testicular sperm aspiration or extraction) – is a surgical procedure where in...
Read MorePGD/PGS
We are happy to announce the availability of the PGD/PGD mentioned tests at Lush Fertility....
Read MoreHysteroscopy
Hysteroscopy is the process of viewing the inside of uterus along with cervical canal. It...
Read MoreLaparoscopy
Laparoscopy is the minimally invasive diagnostic procedure that includes insertion of narrowtelescope camera system to...
Read MoreSurrogacy
Surrogacy is the form of reproductive technology where women offer to carry baby on behalf...
Read MoreIUI – D (Donor)
IUI – D (donor) – is an ART (artificial reproductive technique) procedure where in the...
Read MoreOvulation Induction & Follicular Study With Timely Intercourse
This is a basic step in the treatment of subfertility where medication is given to...
Read MoreCryopreservation
Why cryopreservation? Semen with very limited number of spermatozoa or abnormal semen parameters. Cancer patients...
Read MoreEgg Donation
What does egg donation means? Donor egg program includes oocyte retrieval from healthy young oocyte...
Read MoreIUI (Intra Uterine Insemination)
IUI is one of the simple and basic artificial reproductive techniques used in the treatment...
Read MoreIVF (In Vitro Fertilization)
Commonly known as “Test Tube Baby”, IVF is an advanced technique of artificial reproductive procedures....
Read MoreIVM (in-vitro maturation of oocytes)
This is an advanced technique of IVF where the immature eggs are collected and matured...
Read MoreICSI (Intra cytoplasmic sperm injection)
The donor sperm is used to fertilise the eggs through ICSI intra-cytoplasmic sperm injection) procedure...
Read MoreFresh Embryo Transfer
Once the inseminated oocytes are fertilised, they are cultured in an incubator for a period...
Read MoreFrozen Embryo Transfer
Frozen embryo transfer – When the embryos after 2-5 days of culture are frozen with...
Read MoreBlastocyst Culture
A blastocyst is an embryo that has been developed in laboratory for 5-6 days after...
Read MoreOocyte Freezing
The oocytes (eggs) obtained through ovum pickup procedure are frozen and stored in liquid nitrogen....
Read MoreEmbryo Freezing
This is a procedure where The embryos obtained from ivf procedure are cultured for 2-5...
Read MoreSperm Freezing
Sperm freezing – the sperm samples are washed and stored in liquid nitrogen for later...
Read MoreAdenomyosis
Adenomyosis is a benign gynaecological condition of the uterus where the endometrial tissue i.e the...
Read MoreEndometriosis
Endometriosis is defined as the presence of normal endometrial (uterine lining) tissue in an abnormal...
Read MoreUterine Fibroids( Leiomyoma or Myoma)
Fibroids are benign tumors of the uterine smooth muscle cells. It is estimated that 70-80%...
Read MorePolycystic ovarian syndrome and Polycystic Ovarian disease (PCOD)
Polycystic Ovary Syndrome (PCOS) is a common endocrine disorder, affecting 1 in every 10 women of...
Read More